A HIGHLY ACCURATE NANOPORE-BASED SEQUENCING WORKFLOW FOR CULTURE AND PCR-FREE MICROBIAL METAGENOMIC PROFILING OF UROGENITAL SAMPLES

A highly accurate nanopore-based sequencing workflow for culture and PCR-free microbial metagenomic profiling of urogenital samples

Abstract Background The application of molecular sequencing methods for microbiome profiling of biological samples are largely restricted to research use.However, they require significant resources such as time and cost and can suffer from amplification biases that may hamper interpretation of complex systems.These issues are also a barrier to adop

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Sentiment Analysis of Chinese Ancient Poetry Based on Multidimensional Attention Under the Background of Digital Intelligence Integration

Poetry was a unique literary genre in ancient China as an important way to express sentiments.Chinese ancient poetry not only has simple words, strict meters and rich semantic relationships, but also widely uses rhetorical techniques such as simile and personification, as well as metaphorical means such as allusion and imagery, which makes it diffi

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Giant right coronary artery aneurysm. Case Report

Introduction: Coronary aneurysms are rare and are linked to drug abuse; symptomatology depends on the coronary anatomy.This is a case of acute coronary syndrome associated with a giant right coronary aneurysm.Case description: A 40-year-old male, with history of heroin and crack use since age 20, attended consultation due to dyspnea, stable angina

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